Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously.

ABSTRACT: Allele-specific amplification on the basis of polymerase chain reaction (PCR) has been widely used for single-nucleotide polymorphism (SNP) genotyping. However, the extraction of PCR-compatible genomic DNA from whole blood is usually required. This process is complicated and tedious, and is prone to cause cross-contamination between samples. To facilitate direct PCR amplification from whole blood without the extraction of genomic DNA, we optimized the pH value of PCR solution and the concentrations of magnesium ions and facilitator glycerol. Then, we developed multiplex allele-specific amplifications from whole blood and applied them to a case-control study. In this study, we successfully established triplex, five-plex, and eight-plex allele-specific amplifications from whole blood for det

SUBMITTER: Zhu J 

PROVIDER: S-EPMC3525889 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature