Metabolic potential of a single cell belonging to one of the most abundant lineages in freshwater bacterioplankton.

ABSTRACT: Actinobacteria within the acI lineage are often numerically dominating in freshwater ecosystems, where they can account for >50% of total bacteria in the surface water. However, they remain uncultured to date. We thus set out to use single-cell genomics to gain insights into their genetic make-up, with the aim of learning about their physiology and ecological niche. A representative from the highly abundant acI-B1 group was selected for shotgun genomic sequencing. We obtained a draft genomic sequence in 75 larger contigs (sum=1.16 Mb), with an unusually low genomic G+C mol% (∼42%). Actinobacteria core gene analysis suggests an almost complete genome recovery. We found that the acI-B1 cell had a small genome, with a rather low percentage of genes having no predicted functions (∼15%) as comp

SUBMITTER: Garcia SL 

PROVIDER: S-EPMC3526179 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature