Telomere length homeostasis and telomere position effect on a linear human artificial chromosome are dictated by the genetic background.

ABSTRACT: Telomere position effect (TPE) is the influence of telomeres on subtelomeric epigenetic marks and gene expression. Previous studies suggested that TPE depends on genetic background. As these analyses were performed on different chromosomes, cell types and species, it remains unclear whether TPE represents a chromosome-rather than genetic background-specific regulation. We describe the development of a Linear Human Artificial Chromosome (L-HAC) as a new tool for telomere studies. The L-HAC was generated through the Cre-loxP-mediated addition of telomere ends to an existing circular HAC (C-HAC). As it can be transferred to genetically distinct cell lines and animal models the L-HAC enables the study of TPE in an unprecedented manner. The HAC was relocated to four telomerase-positive cell lin

SUBMITTER: Weuts A 

PROVIDER: S-EPMC3526267 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature