A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network.

ABSTRACT: The epigenetic changes during B-cell development relevant to both normal function and hematologic malignancy are incompletely understood. We examined DNA methylation and RNA expression status during early B-cell development by sorting multiple replicates of four separate stages of pre-B cells derived from normal human fetal bone marrow and applied high-dimension DNA methylation scanning and expression arrays. Features of promoter and gene body DNA methylation were strongly correlated with RNA expression in multipotent progenitors (MPPs) both in a static state and throughout differentiation. As MPPs commit to pre-B cells, a predominantly demethylating phenotype ensues, with 79% of the 2966 differentially methylated regions observed involving demethylation. Demethylation events were more oft

SUBMITTER: Lee ST 

PROVIDER: S-EPMC3526268 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature