Using protein microarray technology to screen anti-ERCC1 monoclonal antibodies for specificity and applications in pathology.

ABSTRACT:

Background

An antibody with cross-reactivity can create unexpected side effects or false diagnostic reports if used for clinical purposes. ERCC1 is being explored as a predictive diagnostic biomarker for cisplatin-based chemotherapy. High ERCC1 expression is linked to drug resistance on cisplatin-based chemotherapy. 8F1 is one of the most commonly used monoclonal antibodies for evaluating ERCC1 expression levels in lung cancer patient tissues, but it has been noted that this antibody cross-reacts with an unknown protein.

Results

By using a high density protein microarray chip technology, we discovered that 8F1 not only reacts with its authentic target, ERCC1, but also cross-reacts with an unrelated nuclear membrane protein, PCYT1A. The cross-reactivity is due to a common epi

SUBMITTER: Ma D 

PROVIDER: S-EPMC3526464 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature