Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5).

ABSTRACT:

Background

The large Glycoside Hydrolase family 5 (GH5) groups together a wide range of enzymes acting on β-linked oligo- and polysaccharides, and glycoconjugates from a large spectrum of organisms. The long and complex evolution of this family of enzymes and its broad sequence diversity limits functional prediction. With the objective of improving the differentiation of enzyme specificities in a knowledge-based context, and to obtain new evolutionary insights, we present here a new, robust subfamily classification of family GH5.

Results

About 80% of the current sequences were assigned into 51 subfamilies in a global analysis of all publicly available GH5 sequences and associated biochemical data. Examination of subfamilies with catalytically-active members revealed that one

SUBMITTER: Aspeborg H 

PROVIDER: S-EPMC3526467 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature