High-throughput analysis of ammonia oxidiser community composition via a novel, amoA-based functional gene array.

ABSTRACT: Advances in microbial ecology research are more often than not limited by the capabilities of available methodologies. Aerobic autotrophic nitrification is one of the most important and well studied microbiological processes in terrestrial and aquatic ecosystems. We have developed and validated a microbial diagnostic microarray based on the ammonia-monooxygenase subunit A (amoA) gene, enabling the in-depth analysis of the community structure of bacterial and archaeal ammonia oxidisers. The amoA microarray has been successfully applied to analyse nitrifier diversity in marine, estuarine, soil and wastewater treatment plant environments. The microarray has moderate costs for labour and consumables and enables the analysis of hundreds of environmental DNA or RNA samples per week per person. T

SUBMITTER: Abell GC 

PROVIDER: S-EPMC3526613 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature