To scavenge or not to scavenge, that is STILL the question.

ABSTRACT: An extensive radiation chemistry literature would suggest that the addition of certain radical scavengers might mitigate the effects of radiation damage during protein crystallography diffraction data collection. However, attempts to demonstrate and quantify such an amelioration and its dose dependence have not yielded consistent results, either at room temperature (RT) or 100 K. Here the information thus far available is summarized and reasons for this lack of quantitative success are identified. Firstly, several different metrics have been used to monitor and quantify the rate of damage, and, as shown here, these can give results which are in conflict regarding scavenger efficacy. In addition, significant variation in results from data collected from crystals treated in nominally the sam

SUBMITTER: Allan EG 

PROVIDER: S-EPMC3526919 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature