CREST maps somatic structural variation in cancer genomes with base-pair resolution.

ABSTRACT: We developed 'clipping reveals structure' (CREST), an algorithm that uses next-generation sequencing reads with partial alignments to a reference genome to directly map structural variations at the nucleotide level of resolution. Application of CREST to whole-genome sequencing data from five pediatric T-lineage acute lymphoblastic leukemias (T-ALLs) and a human melanoma cell line, COLO-829, identified 160 somatic structural variations. Experimental validation exceeded 80%, demonstrating that CREST had a high predictive accuracy.

SUBMITTER: Wang J 

PROVIDER: S-EPMC3527068 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature