Structural insights from random mutagenesis of Campylobacter jejuni oligosaccharyltransferase PglB.

ABSTRACT:

Background

Protein glycosylation is of fundamental importance in many biological systems. The discovery of N-glycosylation in bacteria and the functional expression of the N-oligosaccharyltransferase PglB of Campylobacter jejuni in Escherichia coli enabled the production of engineered glycoproteins and the study of the underlying molecular mechanisms. A particularly promising application for protein glycosylation in recombinant bacteria is the production of potent conjugate vaccines where polysaccharide antigens of pathogenic bacteria are covalently bound to immunogenic carrier proteins.

Results

In this study capsular polysaccharides of the clinically relevant pathogen Staphylococcus aureus serotype 5 (CP5) were expressed in Escherichia coli and linked in vivo to a detoxifie

SUBMITTER: Ihssen J 

PROVIDER: S-EPMC3527161 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature