Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation.

ABSTRACT:

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Background

Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.

Results

New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pron

SUBMITTER: Dehelean CA 

PROVIDER: S-EPMC3527166 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature