Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.

ABSTRACT:

Background

Optimizing the safety and efficacy of standard chemotherapeutic agents such as cisplatin (CDDP) is of clinical relevance. Serum starvation in vitro and short-term food starvation in vivo both stress cells by the sudden depletion of paracrine growth stimulation.

Methods

The effects of serum starvation on CDDP toxicity were investigated in normal and cancer cells by assessing proliferation, cell cycle distribution and activation of DNA-damage response and of AMPK, and were compared to effects observed in cells grown in serum-containing medium. The effects of short-term food starvation on CDDP chemotherapy were assessed in xenografts-bearing mice and were compared to effects on tumor growth and/or regression determined in mice with no diet alteration.

Results

SUBMITTER: Shi Y 

PROVIDER: S-EPMC3527202 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature