Structural analysis of Brucella abortus RicA substitutions that do not impair interaction with human Rab2 GTPase.

ABSTRACT:

Background

Protein-protein interactions are at the basis of many cellular processes, and they are also involved in the interaction between pathogens and their host(s). Many intracellular pathogenic bacteria translocate proteins called effectors into the cytoplasm of the infected host cell, and these effectors can interact with one or several host protein(s). An effector named RicA was recently reported in Brucella abortus to specifically interact with human Rab2 and to affect intracellular trafficking of this pathogen.

Results

In order to identify regions of the RicA protein involved in the interaction with Rab2, RicA was subjected to extensive random mutagenesis using error prone polymerase chain reaction. The resulting allele library was selected by the yeast two-hybrid as

SUBMITTER: Nkengfac B 

PROVIDER: S-EPMC3527289 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature