Bin2 is a membrane sculpting N-BAR protein that influences leucocyte podosomes, motility and phagocytosis.

ABSTRACT: Cell motility, adhesion and phagocytosis are controlled by actin and membrane remodelling processes. Bridging integrator-2 (Bin2) also called Breast cancer-associated protein 1 (BRAP1) is a predicted N-BAR domain containing protein with unknown function that is highly expressed in leucocytic cells. In the present study we solved the structure of Bin2 BAR domain and studied its membrane binding and bending properties in vitro and in vivo. Live-cell imaging experiments showed that Bin2 is associated with actin rich structures on the plasma membrane, where it was targeted through its N-BAR domain. Pull-down experiments and immunoprecipitations showed that Bin2 C-terminus bound SH3 domain containing proteins such as Endophilin A2 and α-PIX. siRNA of endogenous protein led to decreased cell mig

SUBMITTER: Sanchez-Barrena MJ 

PROVIDER: S-EPMC3527510 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature