Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection.

ABSTRACT:

Background

Sapoviruses are single stranded positive sense RNA viruses belonging to the family Caliciviridae. The virus is detected in different species including the human and the porcine species as an enteric pathogen causing asymptomatic to symptomatic enteritis. In this study, we report the development of a rapid real time qRT-PCR based on SYBR Green chemistry for the diagnosis of porcine sapovirus infection in swine.

Results

The method allows the detection of porcine sapoviruses and the quantification of the genomic copies present in stool samples. During its development, the diagnostic tool showed good correlation compared with the gold standard conventional RT-PCR and was ten-fold more sensitive. When the method was applied to field samples, porcine noroviruses from ge

SUBMITTER: Mauroy A 

PROVIDER: S-EPMC3528410 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature