Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea.

ABSTRACT:

Background

While multiple replication origins have been observed in archaea, considerably less is known about their evolutionary processes. Here, we performed a comparative analysis of the predicted (proved in part) orc/cdc6-associated replication origins in 15 completely sequenced haloarchaeal genomes to investigate the diversity and evolution of replication origins in halophilic Archaea.

Results

Multiple orc/cdc6-associated replication origins were predicted in all of the analyzed haloarchaeal genomes following the identification of putative ORBs (origin recognition boxes) that are associated with orc/cdc6 genes. Five of these predicted replication origins in Haloarcula hispanica were experimentally confirmed via autonomous replication activities. Strikingly, several predi

SUBMITTER: Wu Z 

PROVIDER: S-EPMC3528665 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature