Reverse genetics screen in zebrafish identifies a role of miR-142a-3p in vascular development and integrity.

ABSTRACT: MicroRNAs are a well-studied class of non-coding RNA and are known to regulate developmental processes in eukaryotes. Their role in key biological processes such as vasculature development has attracted interest. However, a comprehensive understanding of molecular regulation of angiogenesis and vascular integrity during development remains less explored. Here we identified miRNAs involved in the development and maintenance of vasculature in zebrafish embryos using a reverse genetics approach. Using a combination of bioinformatics predictions and literature based evidences we mined over 701 Human and 329 Zebrafish miRNAs to derive a list of 29 miRNAs targeting vascular specific genes in zebrafish. We shortlisted eight miRNAs and investigated their potential role in regulating vascular devel

SUBMITTER: Lalwani MK 

PROVIDER: S-EPMC3528674 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature