Chemoproteomic profiling identifies changes in DNA-PK as markers of early dengue virus infection.

ABSTRACT: Many cellular factors are regulated via mechanisms affecting protein conformation, localization, and function that may be undetected by most commonly used RNA- and protein-based profiling methods that monitor steady-state gene expression. Mass-spectrometry-based chemoproteomic profiling provides alternatives for interrogating changes in the functional properties of proteins that occur in response to biological stimuli, such as viral infection. Taking dengue virus 2 (DV2) infection as a model system, we utilized reactive ATP- and ADP-acyl phosphates as chemical proteomic probes to detect changes in host kinase function that occur within the first hour of infection. The DNA-dependent protein kinase (DNA-PK) was discovered as a host enzyme with significantly elevated probe labeling within 60

SUBMITTER: Vetter ML 

PROVIDER: S-EPMC3528803 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature