Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells.

ABSTRACT: Recent evidence suggests human embryonic stem cell (hESC) and induced pluripotent stem (iPS) cell lines have differences in their epigenetic marks and transcriptomes, yet the impact of these differences on subsequent terminally differentiated cells is less well understood. Comparison of purified, homogeneous populations of somatic cells derived from multiple independent human iPS and ES lines will be required to address this critical question. Here, we report a differentiation protocol based on embryonic development that consistently yields large numbers of endothelial cells (ECs) derived from multiple hESCs or iPS cells. Mesoderm differentiation of embryoid bodies was maximized, and defined growth factors were used to generate KDR(+) EC progenitors. Magnetic purification of a KDR(+) proge

SUBMITTER: White MP 

PROVIDER: S-EPMC3528812 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature