Peanut oral immunotherapy modifies IgE and IgG4 responses to major peanut allergens.

ABSTRACT:

Background

Patients with peanut allergy have highly stable pathologic antibody repertoires to the immunodominant B-cell epitopes of the major peanut allergens Ara h 1 to 3.

Objective

We used a peptide microarray technique to analyze the effect of treatment with peanut oral immunotherapy (OIT) on such repertoires.

Methods

Measurements of total peanut-specific IgE (psIgE) and peanut-specific IgG(4) (psIgG(4)) were made with CAP-FEIA. We analyzed sera from 22 patients with OIT and 6 control subjects and measured serum specific IgE and IgG(4) binding to epitopes of Ara h 1 to 3 using a high-throughput peptide microarray technique. Antibody affinity was measured by using a competitive peptide microarray, as previously described.

Results

At baseline, psIgE and psIgG(

SUBMITTER: Vickery BP 

PROVIDER: S-EPMC3529994 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature