Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.

ABSTRACT: Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

SUBMITTER: Rossi R 

PROVIDER: S-EPMC3532158 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature