Selective extracellular vesicle-mediated export of an overlapping set of microRNAs from multiple cell types.

ABSTRACT:

Background

MicroRNAs (miRNAs) are a class of small RNA molecules that regulate expression of specific mRNA targets. They can be released from cells, often encapsulated within extracellular vesicles (EVs), and therefore have the potential to mediate intercellular communication. It has been suggested that certain miRNAs may be selectively exported, although the mechanism has yet to be identified. Manipulation of the miRNA content of EVs will be important for future therapeutic applications. We therefore wished to assess which endogenous miRNAs are enriched in EVs and how effectively an overexpressed miRNA would be exported.

Results

Small RNA libraries from HEK293T cells and vesicles before or after transfection with a vector for miR-146a overexpression were analysed by deep se

SUBMITTER: Guduric-Fuchs J 

PROVIDER: S-EPMC3532190 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature