Protein kinase Cε and protein kinase Cθ double-deficient mice have a bleeding diathesis.

ABSTRACT:

Background

In comparison to the classical isoforms of protein kinase C (PKC), the novel isoforms are thought to play minor or inhibitory roles in the regulation of platelet activation and thrombosis.

Objectives

To measure the levels of PKCθ and PKCε and to investigate the phenotype of mice deficient in both novel PKC isoforms.

Methods

Tail bleeding and platelet activation assays were monitored in mice and platelets from mice deficient in both PKCθ and PKCε.

Results

PKCε plays a minor role in supporting aggregation and secretion following stimulation of the collagen receptor GPVI in mouse platelets but has no apparent role in spreading on fibrinogen. PKCθ, in contrast, plays a minor role in supporting adhesion and filopodial generation on fibrinogen but has no a

SUBMITTER: Unsworth AJ 

PROVIDER: S-EPMC3532618 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature