Heterodimerization with the prostacyclin receptor triggers thromboxane receptor relocation to lipid rafts.

ABSTRACT:

Objective

Prostacyclin and thromboxane mediate opposing cardiovascular actions through receptors termed IP and TP, respectively. When dimerized with IP, the TP shifts to IP-like function. IP localizes to cholesterol-enriched membrane rafts, but TP and IPTP heterodimer localization is not defined. We examined these receptors' membrane localization and the role of rafts in receptor function.

Methods and results

Microdomain distribution of IP, TP, and IPTP heterodimers was examined in COS-7 cells by measuring energy transfer from renilla luciferase-fused receptors to fluorescently labeled rafts. IP raft association was confirmed. TP was raft excluded, but redistributed to rafts upon dimerization with IP. Signaling of the IP and IPTP heterodimer, but not TP alone, was suppressed

SUBMITTER: Ibrahim S 

PROVIDER: S-EPMC3533363 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature