Detection of tumor PIK3CA status in metastatic breast cancer using peripheral blood.

ABSTRACT:

Purpose

We sought to evaluate the feasibility of detecting PIK3CA mutations in circulating tumor DNA (ctDNA) from plasma of patients with metastatic breast cancer using a novel technique called BEAMing.

Experimental design

In a retrospective analysis, 49 tumor and temporally matched plasma samples from patients with breast cancer were screened for PIK3CA mutations by BEAMing. We then prospectively screened the ctDNA of 60 patients with metastatic breast cancer for PIK3CA mutations by BEAMing and compared the findings with results obtained by screening corresponding archival tumor tissue DNA using both sequencing and BEAMing.

Results

The overall frequency of PIK3CA mutations by BEAMing was similar in both patient cohorts (29% and 28.3%, respectively). In the retrospect

SUBMITTER: Higgins MJ 

PROVIDER: S-EPMC3533370 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature