Molecular cloning and functional characterization of an ATP-binding cassette transporter OtrC from Streptomyces rimosus.

ABSTRACT:

Background

The otrC gene of Streptomyces rimosus was previously annotated as an oxytetracycline (OTC) resistance protein. However, the amino acid sequence analysis of OtrC shows that it is a putative ATP-binding cassette (ABC) transporter with multidrug resistance function. To our knowledge, none of the ABC transporters in S. rimosus have yet been characterized. In this study, we aimed to characterize the multidrug exporter function of OtrC and evaluate its relevancy to OTC production.

Results

In order to investigate OtrC's function, otrC is cloned and expressed in E. coli The exporter function of OtrC was identified by ATPase activity determination and ethidium bromide efflux assays. Also, the susceptibilities of OtrC-overexpressing cells to several structurally unrelated d

SUBMITTER: Yu L 

PROVIDER: S-EPMC3533511 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature