Beneficial 'unintended effects' of a cereal cystatin in transgenic lines of potato, Solanum tuberosum.

ABSTRACT:

Background

Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects in planta remain poorly understood. Here we assessed the effects of corn cystatin II (CCII), a potent inhibitor of C1A cysteine (Cys) proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein.

Results

The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental) line. Out of ca. 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leave

SUBMITTER: Munger A 

PROVIDER: S-EPMC3534561 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature