RNA-seq and microarray complement each other in transcriptome profiling.

ABSTRACT:

Background

RNA-seq and microarray are the two popular methods employed for genome-wide transcriptome profiling. Current comparison studies have shown that transcriptome quantified by these two methods correlated well. However, none of them have addressed if they complement each other, considering the strengths and the limitations inherent with them. The pivotal requirement to address this question is the knowledge of a well known data set. In this regard, HrpX regulome from pathogenic bacteria serves as an ideal choice as the target genes of HrpX transcription factor are well studied due to their central role in pathogenicity.

Results

We compared the performance of RNA-seq and microarray in their ability to detect known HrpX target genes by profiling the transcriptome from t

SUBMITTER: Kogenaru S 

PROVIDER: S-EPMC3534599 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature