Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

ABSTRACT: Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive technique for gene expression analysis, depends on the stability of the reference genes used for data normalization. Caragana intermedia, a native desert shrub with strong drought-resistance, sand-fixing capacity and high forage value that is widespread in the desert land of west and northwest China, has not been investigated regarding the identification of reference genes suitable for the normalization of qPCR data. In this study, 10 candidate reference genes were analyzed in C. intermedia subjected to different abiotic (osmotic, salt, cold and heat) stresses, in two distinct plant organs (roots and leaves). The expression stability of these genes was assessed using geNorm, NormFinder and BestKeeper

SUBMITTER: Zhu J 

PROVIDER: S-EPMC3534648 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature