Bacterial modulation of human fetal membrane Toll-like receptor expression.

ABSTRACT:

Problem

Preterm premature rupture of fetal membranes (pPROM) occurs in 30-40% of spontaneous preterm births (PTB) and is associated with intra-amniotic infection and inflammation. The membranes may sense and respond to microbes via Toll-like receptors (TLRs); however, little is known about their expression and regulation in this tissue. The objective of this study was to evaluate the expression of TLRs 1-10 in fetal membranes after exposure to pathogens associated with intra-amniotic infection and PTB.

Method of study

Normal human term fetal membrane explants were exposed to various bacteria. After 24 hrs, RNA was extracted and quantitative RT-PCR performed for TLRs1-10.

Results

Treatment of fetal membranes with Mycoplasma hominis increased expression of TLR4, TLR6, a

SUBMITTER: Abrahams VM 

PROVIDER: S-EPMC3535068 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature