A tissue-based comparative effectiveness analysis of biomarkers for early detection of colorectal tumors.

ABSTRACT:

Objectives

We recently identified a six-gene methylation-based biomarker panel suitable for early detection of colorectal cancer (CRC). In this study, we compared the performance of this novel epi-panel with that of previously identified DNA methylation markers in the same clinical tissue sample sets.

Methods

Quantitative methylation-specific PCR was used to analyze the promoter region of SEPT9 and VIM in a total of 485 tissue samples, divided into test and validation sets. ITGA4, NTRK2, OSMR, and TUBG2 were also included in the analyses. Receiver operating characteristic (ROC) curves were used to compare the performances of the individual biomarkers with that of the novel epi-panel.

Results

SEPT9 and VIM were methylated in 82 and 67% of CRCs (n=169) and in 88 and 54%

SUBMITTER: Ahmed D 

PROVIDER: S-EPMC3535074 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature