Microfluidic bioreactor for dynamic regulation of early mesodermal commitment in human pluripotent stem cells.

ABSTRACT: During development and regeneration, tissues emerge from coordinated sequences of stem cell renewal, specialization and assembly that are orchestrated by cascades of regulatory signals. The complex and dynamic in vivo milieu cannot be replicated using standard in vitro techniques. Microscale technologies now offer potential for conducting highly controllable and sophisticated experiments at biologically relevant scales, with real-time insights into cellular responses. We developed a microbioreactor providing time sequences of space-resolved gradients of multiple molecular factors in three-dimensional (3D) cell culture settings, along with a versatile, high-throughput operation and imaging compatibility. A single microbioreactor yields up to 120 data points, corresponding to 15 replicates o

SUBMITTER: Cimetta E 

PROVIDER: S-EPMC3535552 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature