Comparison of sequencing (barcode region) and sequence-tagged-site PCR for Blastocystis subtyping.

ABSTRACT: Blastocystis is the most common nonfungal microeukaryote of the human intestinal tract and comprises numerous subtypes (STs), nine of which have been found in humans (ST1 to ST9). While efforts continue to explore the relationship between human health status and subtypes, no consensus regarding subtyping methodology exists. It has been speculated that differences detected in subtype distribution in various cohorts may to some extent reflect different approaches. Blastocystis subtypes have been determined primarily in one of two ways: (i) sequencing of small subunit rRNA gene (SSU-rDNA) PCR products and (ii) PCR with subtype-specific sequence-tagged-site (STS) diagnostic primers. Here, STS primers were evaluated against a panel of samples (n = 58) already subtyped by SSU-rDNA sequencing (ba

SUBMITTER: Stensvold CR 

PROVIDER: S-EPMC3536234 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature