Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

ABSTRACT:

Background

DNA damage response (DDR) plays pivotal roles in maintaining genome integrity and stability. An effective DDR requires the involvement of hundreds of genes that compose a complicated network. Because DDR is highly conserved in evolution, studies in lower eukaryotes can provide valuable information to elucidate the mechanism in higher organisms. Fission yeast (Schizosaccharomyces pombe) has emerged as an excellent model for DDR research in recent years. To identify novel genes involved in DDR, we screened a genome-wide S. pombe haploid deletion library against six different DNA damage reagents. The library covered 90.5% of the nonessential genes of S. pombe.

Results

We have identified 52 genes that were actively involved in DDR. Among the 52 genes, 20 genes were li

SUBMITTER: Pan X 

PROVIDER: S-EPMC3536581 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature