The proteome of copper, iron, zinc, and manganese micronutrient deficiency in Chlamydomonas reinhardtii.

ABSTRACT: Trace metals such as copper, iron, zinc, and manganese play important roles in several biochemical processes, including respiration and photosynthesis. Using a label-free, quantitative proteomics strategy (MS(E)), we examined the effect of deficiencies in these micronutrients on the soluble proteome of Chlamydomonas reinhardtii. We quantified >10(3) proteins with abundances within a dynamic range of 3 to 4 orders of magnitude and demonstrated statistically significant changes in ~200 proteins in each metal-deficient growth condition relative to nutrient-replete media. Through analysis of Pearson's coefficient, we also examined the correlation between protein abundance and transcript abundance (as determined via RNA-Seq analysis) and found moderate correlations under all nutritional states.

SUBMITTER: Hsieh SI 

PROVIDER: S-EPMC3536910 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature