Characterization of Highper, an ENU-induced mouse mutant with abnormal psychostimulant and stress responses.

ABSTRACT:

Rationale

Chemical mutagenesis in the mouse is a forward genetics approach that introduces random mutations into the genome, thereby providing an opportunity to annotate gene function and characterize phenotypes that have not been previously linked to a given gene.

Objectives

We report on the behavioral characterization of Highper, an N-ethyl-N-nitrosourea (ENU)-induced mutant mouse line.

Methods

Highper and B6 control mice were assessed for locomotor activity in the open field and home cage environments. Basal and acute restraint stress-induced corticosterone levels were measured. Mice were tested for locomotor response to cocaine (5, 20, 30, and 45 mg/kg), methylphenidate (30 mg/kg), and ethanol (0.75, 1.25, and 1.75 g/kg). The rewarding and reinforcing effects of c

SUBMITTER: Eisener-Dorman AF 

PROVIDER: S-EPMC3536991 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature