Cross-study homogeneity of psoriasis gene expression in skin across a large expression range.

ABSTRACT:

Background

In psoriasis, only limited overlap between sets of genes identified as differentially expressed (psoriatic lesional vs. psoriatic non-lesional) was found using statistical and fold-change cut-offs. To provide a framework for utilizing prior psoriasis data sets we sought to understand the consistency of those sets.

Methodology/principal findings

Microarray expression profiling and qRT-PCR were used to characterize gene expression in PP and PN skin from psoriasis patients. cDNA (three new data sets) and cRNA hybridization (four existing data sets) data were compared using a common analysis pipeline. Agreement between data sets was assessed using varying qualitative and quantitative cut-offs to generate a DEG list in a source data set and then using other data sets t

SUBMITTER: Bigler J 

PROVIDER: S-EPMC3537625 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature