Perivascular mural cells of the mouse choroid demonstrate morphological diversity that is correlated to vasoregulatory function.

ABSTRACT:

Objective

Perivascular mural cells of the choroid have been implicated in physiological functioning as well as in retinal disease pathogenesis. However details regarding their form and function are not well understood. We aim to characterize choroidal mural cells in the adult mouse choroid in terms of their distribution and morphology, and correlate these to their contractile behavior.

Methods

Sclerochoroidal flat-mounted explants were prepared from albino transgenic mice in which the α-smooth muscle actin (α-SMA) promoter drives the expression of green fluorescent protein (GFP). α-SMA-expressing smooth muscle cells and pericytes in the living choroid were thereby rendered fluorescent and imaged with confocal microscopy and live-cell imaging in situ.

Results

Choroidal

SUBMITTER: Condren AB 

PROVIDER: S-EPMC3537675 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature