Consensus and pool profiles to assist in the analysis and interpretation of complex low template DNA mixtures.

ABSTRACT: Forensic analysis of low template (LT) DNA mixtures is particularly complicated when (1) LT components concur with high template components, (2) more than three contributors are present, or (3) contributors are related. In this study, we generated a set of such complex LT mixtures and examined two methods to assist in DNA profile analysis and interpretation: the "n/2" consensus method (Benschop et al. 2011) and the pool profile approach. N/2 consensus profiles include alleles that are reproducibly amplified in at least half of the replications. Pool profiles are generated by injecting a blend of independently amplified PCR products on a capillary electrophoresis instrument. Both approaches resulted in a similar increase in the percentage of detected alleles compared to individual profiles,

SUBMITTER: Benschop C 

PROVIDER: S-EPMC3538021 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature