Differential proteomics and physiology of Pseudomonas putida KT2440 under filament-inducing conditions.

ABSTRACT:

Background

Pseudomonas putida exerts a filamentous phenotype in response to environmental stress conditions that are encountered during its natural life cycle. This study assessed whether P. putida filamentation could confer survival advantages. Filamentation of P. putida was induced through culturing at low shaking speed and was compared to culturing in high shaking speed conditions, after which whole proteomic analysis and stress exposure assays were performed.

Results

P. putida grown in filament-inducing conditions showed increased resistance to heat and saline stressors compared to non-filamented cultures. Proteomic analysis showed a significant metabolic change and a pronounced induction of the heat shock protein IbpA and recombinase RecA in filament-inducing conditions

SUBMITTER: Crabbe A 

PROVIDER: S-EPMC3538555 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature