Effects of warm ischemic time on gene expression profiling in colorectal cancer tissues and normal mucosa.

ABSTRACT:

Background

Genome-wide gene expression analyses of tumors are a powerful tool to identify gene signatures associated with biologically and clinically relevant characteristics and for several tumor types are under clinical validation by prospective trials. However, handling and processing of clinical specimens may significantly affect the molecular data obtained from their analysis. We studied the effects of tissue handling time on gene expression in human normal and tumor colon tissues undergoing routine surgical procedures.

Methods

RNA extracted from specimens of 15 patients at four time points (for a total of 180 samples) after surgery was analyzed for gene expression on high-density oligonucleotide microarrays. A mixed-effects model was used to identify probes with differ

SUBMITTER: Musella V 

PROVIDER: S-EPMC3538764 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature