The influence of biomimetic topographical features and the extracellular matrix peptide RGD on human corneal epithelial contact guidance.

ABSTRACT: A major focus in the field of tissue engineering is the regulation of essential cell behaviors through biophysical and biochemical cues from the local extracellular environment. The impact of nanotopographical cues on human corneal epithelial cell (HCEC) contact guidance, proliferation, migration and adhesion have previously been demonstrated. In the current report we have expanded our study of HCEC responses to include both biophysical and controlled biochemical extracellular cues. By exploiting methods for the layer-by-layer coating of substrates with reactive poly(ethylene imine)/poly(2-vinyl-4,4-dimethylazlactone)-based multilayer thin films we have incorporated a single adhesion peptide motif, Arg-Gly-Asp (RGD), on topographically patterned substrates. This strategy eliminates protein

SUBMITTER: Tocce EJ 

PROVIDER: S-EPMC3538935 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature