Forward chemical genetics in yeast for discovery of chemical probes targeting metabolism.

ABSTRACT: The many virtues that made the yeast Saccharomyces cerevisiae a dominant model organism for genetics and molecular biology, are now establishing its role in chemical genetics. Its experimental tractability (i.e., rapid doubling time, simple culture conditions) and the availability of powerful tools for drug-target identification, make yeast an ideal organism for high-throughput phenotypic screening. It may be especially applicable for the discovery of chemical probes targeting highly conserved cellular processes, such as metabolism and bioenergetics, because these probes would likely inhibit the same processes in higher eukaryotes (including man). Importantly, changes in normal cellular metabolism are associated with a variety of diseased states (including neurological disorders and cancer

SUBMITTER: St Onge R 

PROVIDER: S-EPMC3539408 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature