A link between the accumulation of DNA damage and loss of multi-potency of human mesenchymal stromal cells.

ABSTRACT: Human mesenchymal stromal cells (hMSCs) represent an attractive cell source for clinic applications. Besides being multi-potent, recent clinical trials suggest that they secrete both trophic and immunomodulatory factors, allowing allogenic MSCs to be used in a wider variety of clinical situations. The yield of prospective isolation is however very low, making expansion a required step toward clinical applications. Unfortunately, this leads to a significant decrease in their stemness. To identify the mechanism behind loss of multi-potency, hMSCs were expanded until replicative senescence and the concomitant molecular changes were characterized at regular intervals. We observed that, with time of culture, loss of multi-potency was associated with both the accumulation of DNA damage and the r

SUBMITTER: Alves H 

PROVIDER: S-EPMC3539748 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature