Construction and characterization of an in-vivo linear covalently closed DNA vector production system.

ABSTRACT:

Background

While safer than their viral counterparts, conventional non-viral gene delivery DNA vectors offer a limited safety profile. They often result in the delivery of unwanted prokaryotic sequences, antibiotic resistance genes, and the bacterial origins of replication to the target, which may lead to the stimulation of unwanted immunological responses due to their chimeric DNA composition. Such vectors may also impart the potential for chromosomal integration, thus potentiating oncogenesis. We sought to engineer an in vivo system for the quick and simple production of safer DNA vector alternatives that were devoid of non-transgene bacterial sequences and would lethally disrupt the host chromosome in the event of an unwanted vector integration event.

Results

We construct

SUBMITTER: Nafissi N 

PROVIDER: S-EPMC3540006 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature