Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility.

ABSTRACT:

Objective

To determine the effect of infertility-linked sperm phospholipase Cζ (PLCζ) mutations on their ability to trigger oocyte Ca(2+) oscillations and development, and also to evaluate the potential therapeutic utility of wild-type, recombinant PLCζ protein for rescuing failed oocyte activation and embryo development.

Design

Test of a novel therapeutic approach to male factor infertility.

Setting

University medical school research laboratory.

Patient(s)

Donated unfertilized human oocytes from follicle reduction.

Intervention(s)

Microinjection of oocytes with recombinant human PLCζ protein or PLCζ cRNA and a Ca(2+)-sensitive fluorescent dye.

Main outcome measure(s)

Measurement of the efficacy of mutant and wild-type PLCζ-mediated enzyme activit

SUBMITTER: Nomikos M 

PROVIDER: S-EPMC3540263 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature