MicroRNA sequence profiles of human kidney allografts with or without tubulointerstitial fibrosis.

ABSTRACT:

Background

MicroRNA (miRNA) alterations accompanying interstitial fibrosis and tubular atrophy (IFTA) in kidney allografts may point toward pathologic mechanisms. Small-RNA sequencing provides information on total miRNA abundance and specific miRNA expression and allows analysis of differential expression based on read counts.

Methods

MiRNA expression profiles of 8 human kidney allograft biopsies (4 IFTA and 4 normal biopsies, discovery set) were characterized using barcoded deep-sequencing of a cDNA library prepared from multiplexed RNA. Statistical analysis of the sequence data guided selection of miRNAs for validation and the levels of selected miRNAs were quantified in 18 biopsies (10 IFTA and 8 normal) using real-time quantitative PCR assays (RT-QPCR).

Results

To

SUBMITTER: Ben-Dov IZ 

PROVIDER: S-EPMC3541003 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature