Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species.

ABSTRACT:

Background

Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species.

Results

PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed th

SUBMITTER: Stone JK 

PROVIDER: S-EPMC3541218 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature