Drosophila king tubby (ktub) mediates light-induced rhodopsin endocytosis and retinal degeneration.

ABSTRACT:

Background

The tubby (tub) and tubby-like protein (tulp) genes encode a small family of proteins found in many organisms. Previous studies have shown that TUB and TULP genes in mammalian involve in obesity, neural development, and retinal degeneration. The purpose of this study was to investigate the role of Drosophila king tubby (ktub) in rhodopsin 1 (Rh1) endocytosis and retinal degeneration upon light stimulation.

Results

Drosophila ktub mutants were generated using imprecise excision. Wild type and mutant flies were raised in dark or constant light conditions. After a period of light stimulation, retinas were dissected, fixed and stained with anti-Rh1 antibody to reveal Rh1 endocytosis. Confocal and transmission electron microscope were used to examine the retinal degene

SUBMITTER: Chen SF 

PROVIDER: S-EPMC3541268 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature