Involvement of urinary bladder Connexin43 and the circadian clock in coordination of diurnal micturition rhythm.

ABSTRACT: Nocturnal enuresis in children and nocturia in the elderly are two highly prevalent clinical conditions characterized by a mismatch between urine production rate in the kidneys and storage in the urinary bladder during the sleep phase. Here we demonstrate, using a novel method for automated recording of mouse micturition, that connexin43, a bladder gap junction protein, is a negative regulator of functional bladder capacity. Bladder connexin43 levels and functional capacity show circadian oscillations in wild-type mice, but such rhythms are completely lost in Cry-null mice having a dysfunctional biological clock. Bladder muscle cells have an internal clock, and show oscillations of connexin43 and gap junction function. A clock regulator, Rev-erbα, upregulates connexin43 transcription as a

SUBMITTER: Negoro H 

PROVIDER: S-EPMC3541943 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature